14-3-3 KO vector | Addgene 16551 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

14-3-3 KO vector

catalog :

16551

citations: 1

Reference
Chan T, Hermeking H, Lengauer C, Kinzler K, Vogelstein B. 14-3-3Sigma is required to prevent mitotic catastrophe after DNA damage. Nature. 1999;401:616-20 pubmed

product information

Catalog Number :

16551

Product Name :

14-3-3 KO vector

article :

doi	10.1038/44188
id	1856
pubmed_id	10524633

bacterial resistance :

Ampicillin

cloning :

backbone

pBluescript

backbone_mutation

backbone_origin

Stratagene

backbone_size

3000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

A BAC clone was digested with BamHI, and two fragments, one 4.7 kilobases (kb) and the second 3.8 kb, were used to construct the 5' and 3' arms of the targeting vector, respectively. The 4.7-kb subclone contained the region immediately 5' of the initiating codon of the 14-3-3sigma coding region. The 3.8-kb subclone contained a region beginning 900 bp distal to the initiating codon. A synthetic EcoRV site plus the sequence 5'-CGTGGAGAGGGACT GGCAG-3', derived from a region of the 14-3-3sigma locus deleted by the targeting construct, were ligated to the 5' end of a geneticin-resistance gene. This product was placed into the pBluescript plasmid. To facilitate knockout of both alleles, loxP sites surrounding the geneticin-resistance gene were incorporated into the vector.

origin :

pi :

alt_names

14-3-3 sigma

cloning

clone_method	Restriction Enzyme
cloning_site_3	HindIII
cloning_site_5	HindIII
promoter
sequencing_primer_3	T3
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YWHAS
gene	SFN
id	2810

genbank_ids

mutation

name

14-3-3 knockout

shRNA_sequence

size

species

9606

Homo sapiens