This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSIN-TRE-H2BeGFP-rtTA2
catalog :
165494
citations: 1
Reference
Puig I, Tenbaum S, Chicote I, Arqués O, Martinez Quintanilla J, Cuesta Borrás E, et al. TET2 controls chemoresistant slow-cycling cancer cell survival and tumor recurrence. J Clin Invest. 2018;128:3887-3905 pubmed publisher
product information
Catalog Number :
165494
Product Name :
pSIN-TRE-H2BeGFP-rtTA2
article :
doi10.1172/JCI96393
id28215919
pubmed_id29944140
bacterial resistance :
Ampicillin
cloning :
backbonepRRL-cPPT-hPGKTMPrtTA-WPRE
backbone_mutation-
backbone_origin-
backbone_size8023
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth notes :
ABOUT BACKBONE: The lentiviral backbone was originally designed and constructed by Barde I et al. (Barde I et al. Mol Ther. 2006. 13(2): 382-90. PMID: 16275162). ABOUT TEST DIGESTION: Since Xba I restriction site is lost during cloning, we recommend checking the plasmid using the enzymes: Xho I and Nhe I. You should get two fragments around 1600 bp (insert+vector) and 7500 bp (vector).
growth strain :
Doxycycline-inducible all-in-one lentivirus vector to express H2BeGFP fusion protein. System for labelling slow-cycling cells in vivo.
growth temp :
-
origin :
37
pi :
alt_names
-
cloning
clone_methodRestriction Enzyme
cloning_site_3Nhe I
cloning_site_5Xba I
promoterTetO-CMV
sequencing_primer_3TGAAGAATGTGCGAGACC
sequencing_primer_5TCGAGTAGGCGTGTACGGT
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesGL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE
geneH2BC21
id8349
genbank_ids
X00088
mutation-
nameHuman histone H2B
shRNA_sequence
size1116
species
9606
Homo sapiens
tags
locationC terminal on insert
tagEGFP
plasmid copy :
The coding sequence of histone2B fused to eGFP (H2BeGFP) was amplified from the Addgene plasmid ID: 11680 deposited by Geoff Wahl.
resistance markers :
5323
tags :
Unknown
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA