pGST-PPARgamma | Addgene 16549 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGST-PPARgamma

catalog :

16549

citations: 3

Reference
Levan S, Stamnes K, Lin D, Panzer A, Fukui E, McCauley K, et al. Elevated faecal 12,13-diHOME concentration in neonates at high risk for asthma is produced by gut bacteria and impedes immune tolerance. Nat Microbiol. 2019;4:1851-1861 pubmed publisher
Kesanakurti D, Maddirela D, Banasavadi Siddegowda Y, Lai T, Qamri Z, Jacob N, et al. A novel interaction of PAK4 with PPARÎ³ to regulate Nox1 and radiation-induced epithelial-to-mesenchymal transition in glioma. Oncogene. 2017;36:5309-5320 pubmed publisher
He T, Chan T, Vogelstein B, Kinzler K. PPARdelta is an APC-regulated target of nonsteroidal anti-inflammatory drugs. Cell. 1999;99:335-45 pubmed

product information

Catalog Number :

16549

Product Name :

pGST-PPARgamma

article :

doi	10.1016/S0092-8674(00)81664-5
id	1844
pubmed_id	10555149

bacterial resistance :

Ampicillin

cloning :

backbone

pGEX-2TK

backbone_mutation

backbone_origin

Amersham

backbone_size

5000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Note that there are some discrepancies between the insert in this plasmid and the canonical GenBank PPARgamma. The Vogelstein lab maintains that this plasmid worked as specified when they made and used it.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	EcoRI
promoter
sequencing_primer_3	pGEX3'
sequencing_primer_5	pGEX5'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma
gene	PPARG
id	5468

genbank_ids

mutation

N-terminal DNA-binding domain: aa 1-248

name

PPAR gamma

shRNA_sequence

size

744

species

9606

Homo sapiens