This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV3Tag8li-hMBOAT7-3xFLAG
catalog :
165474
citations: 1
Reference
Li S, Wang L, Berman M, Kong Y, Dorf M. Mapping a dynamic innate immunity protein interaction network regulating type I interferon production. Immunity. 2011;35:426-40 pubmed publisher
product information
Catalog Number :
165474
Product Name :
pCMV3Tag8li-hMBOAT7-3xFLAG
article :
doi10.1016/j.immuni.2011.06.014
id4997
pubmed_id21903422
bacterial resistance :
Ampicillin
cloning :
backbonepCMV-3Tag-8 modified MCS
backbone_mutation
backbone_originStratagene /Agilent
backbone_size5200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Backbone contains new MCS (multiple cloning site). CDS without stop codon was inserted with XhoI-NotI and it expresses in frame with 3xFLAG epitope tag. Construct insertion = Xhoi-GCC-[MBOAT7 CDS 1416 bp, no stop]-Noti-A-Clai-GTCGAG-3xFLAG-STOP
growth strain :
Human MBOAT7 mammalian expression vector with 3 C-terminal FLAG tags.
origin :
37
pi :
alt_names
MBOAT7
BC003164.1
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5XhoI
promoterCMV
sequencing_primer_3T7, BGH polyA reverse
sequencing_primer_5CMV, (T3)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBB1, LENG4, LPIAT, LPLAT, LRC4, MBOA7, MRT57, OACT7, hMBOA-7
geneMBOAT7
id79143
genbank_ids
mutation
nameMembrane bound O-acyltransferase domain containing 7
shRNA_sequence
size1416
species
9606
Homo sapiens
tags
locationC terminal on insert
tag3xFLAG
plasmid copy :
Miguel Gijon (JBC 283(44) p30235-.30245)
resistance markers :
120
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA