This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV3Tag8li-hADARB2-3xFLAG
catalog :
165470
citations: 1
Reference
Li S, Wang L, Fu B, Berman M, Diallo A, Dorf M. TRIM65 regulates microRNA activity by ubiquitination of TNRC6. Proc Natl Acad Sci U S A. 2014;111:6970-5 pubmed publisher
product information
Catalog Number :
165470
Product Name :
pCMV3Tag8li-hADARB2-3xFLAG
article :
doi10.1073/pnas.1322545111
id8429
pubmed_id24778252
bacterial resistance :
Ampicillin
cloning :
backbonepCMV-3Tag-8 modified MCS
backbone_mutation
backbone_originStratagene /Agilent
backbone_size5200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
(XhoI-ctg-HindIII-cat-BamHI-aaa-NotI-a-ClaI). CDS without stop codon was inserted into MCS at unkown restriction enzyme sites, and it expresses in frame with 3xFLAG epitope tag. Xhoi was probably the 5' cloning site.
ctcgagctgaagcttcatggatccaaagcggccgcaatc
gat
Backbone contains new MCS (multiple cloning site). MCS is changed to:
growth strain :
Human ADARB2 mammalian expression vector with 3 C-terminal FLAG tags.
origin :
37
pi :
alt_names
ADARB2, RED2, ADAR3
BC137477
cloning
clone_methodRestriction Enzyme
cloning_site_3Unknown
cloning_site_5Unknown
promoterCMV
sequencing_primer_3T7, BGH polyA reverse
sequencing_primer_5CMV, (T3)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesADAR3, RED2
geneADARB2
id105
genbank_ids
mutation
nameAdenosine deaminase RNA specific B2 (inactive)
shRNA_sequence
size2217
species
9606
Homo sapiens
tags
locationC terminal on insert
tag3xFLAG
plasmid copy :
Harvard Institute of Proteomics clone HsCD00342724, MGC:169100
resistance markers :
120
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA