This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV3Tag8li-hSAPS1-HA
catalog :
165466
citations: 1
Reference
Li S, Wang L, Fu B, Berman M, Diallo A, Dorf M. TRIM65 regulates microRNA activity by ubiquitination of TNRC6. Proc Natl Acad Sci U S A. 2014;111:6970-5 pubmed publisher
product information
Catalog Number :
165466
Product Name :
pCMV3Tag8li-hSAPS1-HA
article :
doi10.1073/pnas.1322545111
id8429
pubmed_id24778252
bacterial resistance :
Ampicillin
cloning :
backbonepCMV-3Tag-8 modified MCS
backbone_mutation
backbone_originStratagene /Agilent
backbone_size5200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Backbone contains new multiple cloning site and c-terminal HA epitope instead of FLAGs. Construction: Xhoi-gcc-[SAPS1 CDS 2643 bp, no stop]-Noti-a-Clai-HA epitope tag-ctt-STOP
growth strain :
Human SAPS1 mammalian expression vector with C-terminal HA tag.
origin :
37
pi :
alt_names
PPP6R1, SAPS1
BC111980; NM_014931.3
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5XhoI
promoterCMV
sequencing_primer_3T7, BGH polyA reverse
sequencing_primer_5CMV, (T3)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesKIAA1115, PP6R1, SAP190, SAPS1
genePPP6R1
id22870
genbank_ids
mutation
nameProtein phosphatase 6 regulatory subunit 1
shRNA_sequence
size2643
species
9606
Homo sapiens
tags
locationC terminal on insert
tagHA
plasmid copy :
Harvard Institute of Proteomics clone HsCD00341883, MGC:138185IMAGE ID 8327448.
resistance markers :
120
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA