This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
StrKDEL-IRES-mannosidase II-mTagBFP2
catalog :
165460
citations: 1
Reference
McCaughey J, Stevenson N, Mantell J, Neal C, Paterson A, Heesom K, et al. A general role for MIA3/TANGO1 in secretory pathway organization and function. J Cell Sci. 2021;: pubmed publisher
product information
Catalog Number :
165460
Product Name :
StrKDEL-IRES-mannosidase II-mTagBFP2
article :
doi10.1242/jcs.259075
id28215910
pubmed_id34350936
bacterial resistance :
Ampicillin
cloning :
backbonepIRESneo3
backbone_mutation
backbone_originClontech
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
This plasmid is a derivative of StrKDEL-IRES-ManII-mScarlet-i (Plasmid #117274)
growth strain :
Expressed mannII-BFP simultaneously with a RUSH Hook Str-KDEL.
origin :
37
pi :
alt_names
This contains only amino acids 1 to 116 of ManII
cloning
clone_methodRestriction Enzyme
cloning_site_3FseI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMa, Man, Mana-2, Mana2, Map, Map-2
geneMan2a1
id17158
genbank_ids
NM_008549.2
mutationcontains only amino acids 1 to 116 of ManII
namePartial ManII-mTag2BFP fusion
shRNA_sequence
size
species
10090
Mus musculus
tags
locationC terminal on insert
tagmTag2BFP
locationN terminal on backbone
tagStr-KDEL
plasmid copy :
Original clone was Plasmid #65253 from Franck Perez. mScarlet-i was synthesized from published sequence.
resistance markers :
2402
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA