This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSico_U6-PGC1a1 sgRNA
catalog :
165425
citations: 1
Reference
Musicant A, Parag Sharma K, Gong W, Sengupta M, Chatterjee A, Henry E, et al. CRTC1/MAML2 directs a PGC-1α-IGF-1 circuit that confers vulnerability to PPARγ inhibition. Cell Rep. 2021;34:108768 pubmed publisher
product information
Catalog Number :
165425
Product Name :
pSico_U6-PGC1a1 sgRNA
article :
doi10.1016/j.celrep.2021.108768
id28211480
pubmed_id33626346
bacterial resistance :
Ampicillin
cloning :
backbonepSico
backbone_mutationmKate2-T2A-Bsd cassette included on backbone driven by CAGs promoter
backbone_origin
backbone_size9580
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
CRISPR
growth strain :
Expression of gRNA against human PGC-1a variant 1
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Esp3I
cloning_site_5Esp3I
promoter
sequencing_primer_3GATCTACCACATTTGTAGAG
sequencing_primer_5CCTCTGCTAACCATGTTCATGC
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesLEM6, PGC-1(alpha), PGC-1alpha, PGC-1v, PGC1, PGC1A, PPARGC1
genePPARGC1A
id10891
genbank_ids
NM_001354825
mutation
namegRNA against PGC-1a variant 1
shRNA_sequenceAC TGG GGA CTG TAG TAA GAC
size
species
9606
Homo sapiens
100
Synthetic
tags
resistance markers :
2606
tags :
High Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA