This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDDGFP-Leu2d_GgPCFT
catalog :
165414
citations: 1
Reference
Parker J, Deme J, Kuteyi G, Wu Z, Huo J, Goldman I, et al. Structural basis of antifolate recognition and transport by PCFT. Nature. 2021;595:130-134 pubmed publisher
product information
Catalog Number :
165414
Product Name :
pDDGFP-Leu2d_GgPCFT
article :
doi10.1038/s41586-021-03579-z
id28215892
pubmed_id34040256
bacterial resistance :
Ampicillin
cloning :
backbonepDDGFP_LEU2d
backbone_mutation
backbone_origin
backbone_size8654
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth strain :
expression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterGAL1
sequencing_primer_3agaaaatttgtgaccattaac
sequencing_primer_5cttcttattcaaatgtaa
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPCFT
geneSLC46A1
id417569
genbank_ids
mutation
nameGgPCFT
shRNA_sequence
size1419
species
9031
Gallus gallus
100
Synthetic
tags
locationC terminal on backbone
tagGFP-His8
resistance markers :
2005
tags :
High Copy
terms :
LEU2
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA