BDS-2 3x MUT (p53 binding site) | Addgene 16516 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

BDS-2 3x MUT (p53 binding site)

catalog :

16516

citations: 1

Reference
Hermeking H, Lengauer C, Polyak K, He T, Zhang L, Thiagalingam S, et al. 14-3-3sigma is a p53-regulated inhibitor of G2/M progression. Mol Cell. 1997;1:3-11 pubmed

product information

Catalog Number :

16516

Product Name :

BDS-2 3x MUT (p53 binding site)

article :

doi	10.1016/S1097-2765(00)80002-7
id	1851
pubmed_id	9659898

bacterial resistance :

Ampicillin

cloning :

backbone

pBV-Luc

backbone_mutation

backbone_origin

Vogelstein Lab

backbone_size

4900

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

This reporter contains 3 copies of the mutant p53-binding site from the 14-3-3 sigma promoter. The following oligonucleotide pairs were used: 5'-CCTGTAGAAT TATCCCAGAA ATTTCCCTAC TCCGTAC-3' and 5'-GGAGTAGGGA AATTTCTGGG ATAATTCTAC AGGGTAC-3' for BDS-2*, altered at critical p53-binding residues.

origin :

pi :

alt_names

14-3-3 sigma promoter

cloning

clone_method	Restriction Enzyme
cloning_site_3	KpnI
cloning_site_5	KpnI
promoter
sequencing_primer_3	LucNrev
sequencing_primer_5	RVprimer3
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YWHAS
gene	SFN
id	2810

genbank_ids

mutation

Mutant p53 binding sites

name

p53 binding site

shRNA_sequence

size

species

9606

Homo sapiens