This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
ORF3a-EGFP
catalog :
165121
citations: 1
Reference
Miserey Lenkei S, Trajkovic K, D Ambrosio J, Patel A, Copic A, Mathur P, et al. A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterisation in different cell types. Biol Cell. 2021;113:311-328 pubmed publisher
product information
Catalog Number :
165121
Product Name :
ORF3a-EGFP
article :
doi10.1111/boc.202000158
id28215876
pubmed_id33666950
bacterial resistance :
Kanamycin
cloning :
backbonepEGFPN1
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
growth strain :
mammalian expression and localization
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NheI
promoterCMV
sequencing_primer_3CGTCCAGCTCGACCAGGATG
sequencing_primer_5GTCGTAACAACTCCGCCC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGU280_gp03
geneORF3a
id43740569
genbank_ids
mutation
nameORF3a
shRNA_sequence
size800
species
32644
Other
codon optimized SARS-COV-2
tags
locationC terminal on insert
tagEGFP
plasmid copy :
Inserts were prepared by PCR with templates that have been published elsewhere: Gordon et al., 2020a.
resistance markers :
5307
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA