This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX4T-3-6AOptParkin
catalog :
165107
product information
Catalog Number :
165107
Product Name :
pGEX4T-3-6AOptParkin
article :
doi
id28215864
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepGEX4T-3
backbone_mutation
backbone_originGE Healthcare
backbone_size4900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C.
growth strain :
bacterial expression of a GST fusion of e.coli optimized Parkin with 6 alanine linker between the GST and the inserted Parkin gene
origin :
37
pi :
alt_names
PARK2
cloning
clone_methodRestriction Enzyme
cloning_site_3Not1
cloning_site_5Sal1
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAR-JP, LPRS2, PARK2, PDJ
genePRKN
id5071
genbank_ids
mutationcodon optimized for E. coli expression
nameParkin isoform 1
shRNA_sequence
size1413
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST-6-alanine
resistance markers :
5309
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA