This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX4T1-ISG15 C78SN13Y
catalog :
165106
citations: 1
Reference
Narasimhan J, Wang M, Fu Z, Klein J, Haas A, KIM J. Crystal structure of the interferon-induced ubiquitin-like protein ISG15. J Biol Chem. 2005;280:27356-65 pubmed
product information
Catalog Number :
165106
Product Name :
pGEX4T1-ISG15 C78SN13Y
article :
doi10.1074/jbc.M502814200
id28215870
pubmed_id15917233
bacterial resistance :
Ampicillin
cloning :
backbonepGEX4T-1
backbone_mutation
backbone_originGE Healthcare
backbone_size4900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
bacterial expressio of a GST fusion of Human ISG15 C78SN13Y
origin :
37
pi :
alt_names
UCRP
cloning
clone_methodRestriction Enzyme
cloning_site_3Xho1
cloning_site_5BamH1
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesG1P2, IFI15, IMD38, IP17, UCRP, hUCRP
geneISG15
id9636
genbank_ids
mutationC78SN13Y; The sequence of ISG15-C78S/N13Y contains a cysteine to serine mutation at residue 78 which has been found to increase stability of the protein during purification, concentration, and storage. It also contains an asparagine to tyrosine mutation at residue 13 to produce a solvent accessible site for labeling with 125Iodine
nameISG15
shRNA_sequence
size471
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGST from plasmid.
resistance markers :
5309
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA