This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX6P1-optimizedNedd4.2F823D
catalog :
165105
citations: 1
Reference
Todaro D, Augustus Wallace A, Klein J, Haas A. Oligomerization of the HECT ubiquitin ligase NEDD4-2/NEDD4L is essential for polyubiquitin chain assembly. J Biol Chem. 2018;293:18192-18206 pubmed publisher
product information
Catalog Number :
165105
Product Name :
pGEX6P1-optimizedNedd4.2F823D
article :
doi10.1074/jbc.RA118.003716
id28215869
pubmed_id30287686
bacterial resistance :
Ampicillin
cloning :
backbonepGEX6P1
backbone_mutation
backbone_originGE Healthcare
backbone_size4900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C. 5' Cloning Site: BamHI (not destroyed), 3' Cloning Site: EcoRI (not destroyed).
growth strain :
bacterial expression of a GST fusion of Human Nedd4.2d F823D
origin :
37
pi :
alt_names
NEDD4L
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNEDD4-2, NEDD4.2, PVNH7, RSP5, hNEDD4-2
geneNEDD4L
id23327
genbank_ids
mutationcodon optimized for E. coli expression; F823D; mutation for destabilization of trimer formation
nameNedd4.2d F823D
shRNA_sequence
size2868
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGST from plasmid.
resistance markers :
5309
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA