This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX6P1-optimizedNedd4.2d
catalog :
165103
citations: 1
Reference
Todaro D, Augustus Wallace A, Klein J, Haas A. The mechanism of neural precursor cell expressed developmentally down-regulated 4-2 (Nedd4-2)/NEDD4L-catalyzed polyubiquitin chain assembly. J Biol Chem. 2017;292:19521-19536 pubmed publisher
product information
Catalog Number :
165103
Product Name :
pGEX6P1-optimizedNedd4.2d
article :
doi10.1074/jbc.M117.817882
id28215868
pubmed_id28972136
bacterial resistance :
Ampicillin
cloning :
backbonepGEX6P1
backbone_mutation
backbone_originGE Healthcare
backbone_size4900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C.
growth strain :
bacterial expression of a GST fusion of Human Nedd4.2d
origin :
37
pi :
alt_names
NEDD4L
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoR1
cloning_site_5BamH1
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNEDD4-2, NEDD4.2, PVNH7, RSP5, hNEDD4-2
geneNEDD4L
id23327
genbank_ids
mutationcodon optimized for E. coli expression
nameNedd4.2d
shRNA_sequence
size2868
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGST from plasmid.
resistance markers :
5309
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA