This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX-E6AP Hect
catalog :
165102
citations: 1
Reference
Ronchi V, Haas A. Measuring rates of ubiquitin chain formation as a functional readout of ligase activity. Methods Mol Biol. 2012;832:197-218 pubmed publisher
product information
Catalog Number :
165102
Product Name :
pGEX-E6AP Hect
article :
doi10.1007/978-1-61779-474-2_14
id28215867
pubmed_id22350887
bacterial resistance :
Ampicillin
cloning :
backbonepGEX4T-3
backbone_mutation
backbone_originGE Healthcare
backbone_size4900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C.
growth strain :
bacterial expression of a GST fusion of Human E6AP Hect domain
origin :
37
pi :
alt_names
UBE3A Hect
cloning
clone_methodRestriction Enzyme
cloning_site_3Xho1
cloning_site_5EcoR1
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesANCR, AS, E6-AP, EPVE6AP, HPVE6A, PIX1
geneUBE3A
id7337
genbank_ids
mutationE6AP Hect domain Amino acids 495-738 (5'QLNPY-LICG3')
nameE6AP Hect
shRNA_sequence
size731
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGST from plasmid.
resistance markers :
5309
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA