pGEX-optimized E6AP F727D | Addgene 165101 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGEX-optimized E6AP F727D

catalog :

165101

citations: 1

Reference
Ronchi V, Klein J, Edwards D, Haas A. The active form of E6-associated protein (E6AP)/UBE3A ubiquitin ligase is an oligomer. J Biol Chem. 2014;289:1033-48 pubmed publisher

product information

Catalog Number :

165101

Product Name :

pGEX-optimized E6AP F727D

article :

doi	10.1074/jbc.M113.517805
id	28215866
pubmed_id	24273172

bacterial resistance :

Ampicillin

cloning :

backbone

pGEX4T1

backbone_mutation

backbone_origin

GE Healthcare

backbone_size

4900

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Amino acid numbering is based on Isoform 1 though this clone is isoform 3. The sequence is 5'-SPLKYLFRPEE-3' where F has been mutated to D. Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C. 5' Cloning Site: EcoRI (not destroyed), 3' Cloning Site: XhoI (not destroyed).

growth strain :

ecoli optimized expression of a GST fusion of Human E6AP F727D

origin :

pi :

alt_names

UBE3A

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ANCR, AS, E6-AP, EPVE6AP, HPVE6A, PIX1
gene	UBE3A
id	7337

genbank_ids

mutation

codon optimized for E. coli expression

name

E6AP F727D

shRNA_sequence

size

2620

species

9606

Homo sapiens