This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX4T-1-6AlanineHE1
catalog :
165098
product information
Catalog Number :
165098
Product Name :
pGEX4T-1-6AlanineHE1
article :
doi
id28215864
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepGEX4T1
backbone_mutation
backbone_originGE Healthcare
backbone_size4900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C.
growth strain :
bacterial expression of a GST fusion of Human Uba1 with a 6alanine linker between the GST encoded by the plasmid and the inserted Uba1
origin :
37
pi :
alt_names
UBA1A
cloning
clone_methodRestriction Enzyme
cloning_site_3Not1
cloning_site_5BamH1
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesA1S9, A1S9T, A1ST, AMCX1, CFAP124, GXP1, POC20, SMAX2, UBA1A, UBE1, UBE1X, VEXAS
geneUBA1
id7317
genbank_ids
mutationNone
name Uba1
shRNA_sequence
size3195
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST-6-alanine
resistance markers :
5309
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA