This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET19b-EcMscL-TEV-mEGFP
catalog :
165097
citations: 1
Reference
Jacobs M, Boyd M, Kamat N. Diblock copolymers enhance folding of a mechanosensitive membrane protein during cell-free expression. Proc Natl Acad Sci U S A. 2019;: pubmed publisher
product information
Catalog Number :
165097
Product Name :
pET19b-EcMscL-TEV-mEGFP
article :
doi10.1073/pnas.1814775116
id28204678
pubmed_id30760590
bacterial resistance :
Ampicillin
cloning :
backbonepET19b
backbone_mutation
backbone_origin
backbone_size5600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Synthetic Biology
growth strain :
Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.
origin :
37
pi :
alt_names
MscLGFP
EcMscL-mEGFP
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NdeI
promoterT7
sequencing_primer_3T7 terminator
sequencing_primer_5T7 fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb3291, ECK3277, yhdC
genemscL
id947787
genbank_ids
mutation
nameE. Coli Mechanosensitive Channel of Large Conductance
shRNA_sequence
size1236
species
32644
Other
E. coli
tags
locationC terminal on insert
tagmonomeric Enhanced Green Fluorescent Protein
resistance markers :
5310
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA