This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMasterK
catalog :
165081
citations: 1
Reference
Yoshimatsu S, Qian E, Sato T, Yamamoto M, Ishikawa M, Okano H. Establishing an induced pluripotent stem cell line from neonatal common marmoset fibroblasts by an all-in-one episomal vector approach. Stem Cell Res. 2021;53:102380 pubmed publisher
product information
Catalog Number :
165081
Product Name :
pMasterK
article :
doi10.1016/j.scr.2021.102380
id28215860
pubmed_id34088009
bacterial resistance :
Ampicillin
cloning :
backbonepMaster
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
Episomal
growth notes :
Please note that this plasmid contains repetitive elements which are prone to recombination. Screen 3-5 small colonies to ensure the full plasmid is selected. For example, the restriction enzyme NheI can be used to check the plasmid size as shown in Addgene's Diagnostic Digest.
growth strain :
Episomal expression of reprogramming factors.
growth temp :
Plasmid is unstable and prone to recombination. Test 3-5 small colonies to ensure the full plasmid is intact.
origin :
37
pi :
alt_names
POU5F1
cloning
entrez_gene
aliasesOCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
genePOU5F1
id5460
genbank_ids
mutation
nameOCT4
shRNA_sequence
size1080
species
9606
Homo sapiens
tags
alt_names
cloning
entrez_gene
aliasesANOP3, MCOPS3
geneSOX2
id6657
genbank_ids
mutation
nameSOX2
shRNA_sequence
size951
species
9606
Homo sapiens
tags
alt_names
cloning
entrez_gene
aliasesEZF, GKLF
geneKLF4
id9314
genbank_ids
mutation
nameKLF4
shRNA_sequence
size1410
species
9606
Homo sapiens
tags
alt_names
C-MYC
cloning
entrez_gene
aliasesMRTL, MYCC, bHLHe39, c-Myc
geneMYC
id4609
genbank_ids
mutation
nameMYC
shRNA_sequence
size1320
species
9606
Homo sapiens
tags
alt_names
cloning
entrez_gene
aliasesB1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2
geneNR5A2
id2494
genbank_ids
mutation
nameLRH1
shRNA_sequence
size1488
species
9606
Homo sapiens
tags
alt_names
cloning
entrez_gene
aliases
geneNANOG
id79923
genbank_ids
mutation
nameNANOG
shRNA_sequence
size915
species
9606
Homo sapiens
tags
alt_names
cloning
entrez_gene
aliasesCSDD1, LIN-28, LIN28, ZCCHC1, lin-28A
geneLIN28A
id79727
genbank_ids
mutation
namelin28
shRNA_sequence
size627
species
9606
Homo sapiens
tags
alt_names
cloning
entrez_gene
genbank_ids
mutation
nameEGFP
shRNA_sequence
size710
species
32644
Other
Aequorea victoria
tags
alt_names
cloning
entrez_gene
genbank_ids
mutation
nameHSV TK
shRNA_sequence
size1131
species
32644
Other
Herpes Simplex Virus
tags
alt_names
cloning
entrez_gene
aliasesJMJD2D
geneKDM4D
id55693
genbank_ids
mutation
nameKDM4D
shRNA_sequence
size1569
species
32644
Other
Callithrix jacchus
tags
alt_names
cloning
entrez_gene
aliases
geneGLIS1
id148979
genbank_ids
mutation
nameGLIS1
shRNA_sequence
size1860
species
9606
Homo sapiens
tags
resistance markers :
4567
tags :
High Copy
terms :
Neomycin (select with G418)
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA