This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLJM1-Flag-Rheb-4Rs
catalog :
165022
citations: 1
Reference
Yao Y, Hong S, Ikeda T, Mori H, MacDougald O, Nada S, et al. Amino Acids Enhance Polyubiquitination of Rheb and Its Binding to mTORC1 by Blocking Lysosomal ATXN3 Deubiquitinase Activity. Mol Cell. 2020;80:437-451.e6 pubmed publisher
product information
Catalog Number :
165022
Product Name :
pLJM1-Flag-Rheb-4Rs
article :
doi10.1016/j.molcel.2020.10.004
id28215848
pubmed_id33157014
bacterial resistance :
Ampicillin
cloning :
backbonepLJM1
backbone_mutation
backbone_originAvailable at Addgene (#19319)
backbone_size7400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
expression of Flag-Rheb-4Rs mutant protein in mammalian cells (the lysine residues on the position 109, 135, 151 and 178 on Rheb protein are mutated to arginines)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5SalI
promoter
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesRHEB2
geneRHEB
id6009
genbank_ids
NM_005614.4
mutationchange lysine residues on position 109, 135, 151, 178 to arginines
nameRheb-4Rs
shRNA_sequence
size555
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagflag
resistance markers :
5315
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA