This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLVX-NPC1(F503/4A)-FLAG
catalog :
164974
citations: 1
Reference
Davis O, Shin H, Lim C, Wu E, Kukurugya M, Maher C, et al. NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C. Dev Cell. 2021;56:260-276.e7 pubmed publisher
product information
Catalog Number :
164974
Product Name :
pLVX-NPC1(F503/4A)-FLAG
article :
doi10.1016/j.devcel.2020.11.016
id28215845
pubmed_id33308480
bacterial resistance :
Ampicillin
cloning :
backbonepLVX-AcGFP-N1
backbone_mutation
backbone_originClontech
backbone_size9519
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Lentiviral vector for NPC1 expression in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XmaI
cloning_site_5EcoRI
promoterEF1a
sequencing_primer_3CATGCTCCAGACTGCCTTG
sequencing_primer_5TCAAGCCTCAGACAGTGGTTC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNPC, POGZ, SLC65A1
geneNPC1
id4864
genbank_ids
mutationF503A and F504A
nameNPC1
shRNA_sequence
size3867
species
9606
Homo sapiens
tags
locationC terminal on insert
tagFLAG
resistance markers :
4004
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA