This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pENTR-D-TOPO-kcnn1a
catalog :
164956
citations: 1
Reference
Silic M, Black M, Zhang G. Phylogenetic and developmental analyses indicate complex functions of calcium-activated potassium channels in zebrafish embryonic development. Dev Dyn. 2021;: pubmed publisher
product information
Catalog Number :
164956
Product Name :
pENTR-D-TOPO-kcnn1a
article :
doi10.1002/dvdy.329
id28215840
pubmed_id33728688
bacterial resistance :
Kanamycin
cloning :
backbonepENTR-D-TOPO
backbone_mutation
backbone_originInvitrogen
backbone_size2580
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
PCR cloning vector
growth notes :
before the start codon. But this will not impact the result of the whole mount in situ hybridization.
(ATGCGGCATCGGCACGCGGGCCATGCGGCATCGGCACG
CGGGCC)
The DNA sequence was amplified from pooled wild-type TAB zebrafish embryos. According to the predicted sequence, XP_005171293.1, there are likely polymorphisms, K185E, H323Y, S599N, which are not listed in the current ENSEMBL database. This is possibly due to incomplete zebrafish genome annotation, GRCz11. In addition, at the 5 end of the insert, there are 44bps extra forward primer dimer sequences
growth strain :
Zebrafish kcnn1a gene CDS
origin :
37
pi :
alt_names
si:rp71-39b20.3
cloning
clone_methodTOPO Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13R
sequencing_primer_5M13F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasessi:rp71-39b20.3
genekcnn1a
id563352
genbank_ids
mutation
namekcnn1a
shRNA_sequence
size2673
species
7955
Danio rerio
tags
resistance markers :
4313
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA