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product summary
company name :
Addgene
product type :
cDNA
product name :
pInducer10b-HA-KRAS G12V CO SR
catalog :
164928
citations: 1
Reference
Yuan T, Fellmann C, Lee C, Ritchie C, Thapar V, Lee L, et al. Development of siRNA payloads to target KRAS-mutant cancer. Cancer Discov. 2014;4:1182-1197 pubmed publisher
product information
Catalog Number :
164928
Product Name :
pInducer10b-HA-KRAS G12V CO SR
article :
doi10.1158/2159-8290.CD-13-0900
id28215836
pubmed_id25100204
bacterial resistance :
Ampicillin
cloning :
backbonepInducer-10b
backbone_mutation
backbone_origin
backbone_size12168
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth strain :
Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRASG12V sequence
origin :
37
pi :
alt_names
KRAS
cloning
clone_methodRestriction Enzyme
cloning_site_3Mlu I
cloning_site_5AgeI
promoterUbc promoter
sequencing_primer_3ctgatccttccgcccggacgctcag
sequencing_primer_5ctaccggtagatctaccatg
site_3_destroyed
site_5_destroyed
entrez_gene
aliases'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2
geneKRAS
id3845
genbank_ids
NM_004985.5
mutationGlycine 12 changed to valine; 9 Valine codons are optimized and siRNA targeting sites are altered without effecting amino acid codons.
nameKRAS proto-oncogene, GTPase (KRAS)
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA tag
resistance markers :
5444
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA