This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pInducer10b-EGFP-KRAS G12V
catalog :
164925
citations: 2
Reference
Pond K, Morris J, Alkhimenok O, Varghese R, Cabel C, Ellis N, et al. Live-cell imaging in human colonic monolayers reveals ERK waves limit the stem cell compartment to maintain epithelial homeostasis. elife. 2022;11: pubmed publisher
Carver J, Dexheimer T, Hsu D, Weng M, Smith J, Guha R, et al. A high-throughput assay for small molecule destabilizers of the KRAS oncoprotein. PLoS ONE. 2014;9:e103836 pubmed publisher
product information
Catalog Number :
164925
Product Name :
pInducer10b-EGFP-KRAS G12V
article :
doi10.1371/journal.pone.0103836
id28215835
pubmed_id25093678
bacterial resistance :
Ampicillin
cloning :
backbonepInducer-10b
backbone_mutation
backbone_origin
backbone_size12176
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth strain :
Expresses EGFP cDNA and KRAS G12V cDNA in mammalian cells
origin :
37
pi :
alt_names
EGFP
cloning
clone_methodRestriction Enzyme
cloning_site_3Not I
cloning_site_5AgeI
promoterUbc promoter
sequencing_primer_3
sequencing_primer_5ACCATGGTGAGCAAGGGCG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameEnhanced green fluorescent protein
shRNA_sequence
size717
species
100
Synthetic
tags
alt_names
KRAS G12V
cloning
clone_methodRestriction Enzyme
cloning_site_3Mlu I
cloning_site_5AgeI
promoterUbc Promoter
sequencing_primer_3CGAGAAGCGCGATCACATGGTCCTGCTGG
sequencing_primer_5ATGACTGAATATAAACTTGT
site_3_destroyed
site_5_destroyed
entrez_gene
aliases'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2
geneKRAS
id3845
genbank_ids
NM_004985.5
mutationGlycine 12 changed to valine (G->T 3156)
nameKRAS proto-oncogene, GTPase (KRAS) G12V mutant
shRNA_sequence
size567
species
9606
Homo sapiens
tags
resistance markers :
5444
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA