This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pU6-mir451 EF1Alpha-puro-T2A-BFP
catalog :
164792
citations: 1
Reference
Fang W, Bartel D. MicroRNA Clustering Assists Processing of Suboptimal MicroRNA Hairpins through the Action of the ERH Protein. Mol Cell. 2020;78:289-302.e6 pubmed publisher
product information
Catalog Number :
164792
Product Name :
pU6-mir451 EF1Alpha-puro-T2A-BFP
article :
doi10.1016/j.molcel.2020.01.026
id28213934
pubmed_id32302541
bacterial resistance :
Ampicillin
cloning :
backbonepU6-sgRNA EF1Alpha-puro-T2A-BFP
backbone_mutation
backbone_originJonathan Weissman (Addgene plasmid # 60955)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Express miR-451 under the U6 promoter in mammalian cells
origin :
30
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BstXI
promoterU6
sequencing_primer_3
sequencing_primer_5U6_seq
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIR451, MIRN451, hsa-mir-451, hsa-mir-451a, mir-451a
geneMIR451A
id574411
genbank_ids
mutation
namemicroRNA 451a
shRNA_sequence
size
species
9606
Homo sapiens
tags
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterEF1Alpha
sequencing_primer_3
sequencing_primer_5EF1Alpha sequencing primer
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namepuro-T2A-BFP
shRNA_sequence
size
species
tags
resistance markers :
21
tags :
Low Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA