This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pX459-CTNNB1-S45
catalog :
164587
citations: 1
Reference
de Man S, Zwanenburg G, van der Wal T, Hink M, van Amerongen R. Quantitative live-cell imaging and computational modeling shed new light on endogenous WNT/CTNNB1 signaling dynamics. elife. 2021;10: pubmed publisher
product information
Catalog Number :
164587
Product Name :
pX459-CTNNB1-S45
article :
doi10.7554/eLife.66440
id28211397
pubmed_id34190040
bacterial resistance :
Ampicillin
cloning :
backbonepSpCas9(BB)-2A-Puro (PX459) V2.0 (Feng Zhang, Plasmid #62988)
backbone_mutation
backbone_origin
backbone_size9158
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/2020.05.28.120543v1 for bioRxiv preprint.
growth strain :
Expresses a gRNA that overlaps the S45 codon of human CTNNB1
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BbsI
cloning_site_5BbsI
promoterU6
sequencing_primer_3
sequencing_primer_5U6 FWD
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesCTNNB, EVR7, MRD19, NEDSDV, armadillo
geneCTNNB1
id1499
genbank_ids
mutation
nameCTNNB1 gRNA
shRNA_sequenceTTGCCTTTACCACTCAGAGA
size
species
9606
Homo sapiens
tags
resistance markers :
4554
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA