This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GP825
catalog :
164550
citations: 1
Reference
Desai P, Stuck M, Lv B, Pazour G. Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling. J Cell Biol. 2020;219: pubmed publisher
product information
Catalog Number :
164550
Product Name :
GP825
article :
doi10.1083/jcb.201912104
id28215746
pubmed_id32435793
bacterial resistance :
Ampicillin
cloning :
backbonePhage lentivirus
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth strain :
Expresses Smo-FLAG K434, 448R
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterGgCryD1
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesE130215L21Rik, Smoh, bnb, smoothened
geneSmo
id319757
genbank_ids
mutationTwo of the three Lysines in loop three of Smoothened changed to arginine (KKK to RKR). K434, 448R
nameSmoothened K434, 448R
shRNA_sequence
size
species
10090
Mus musculus
tags
locationC terminal on insert
tagFLAG
resistance markers :
1572
tags :
High Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA