GP805 | Addgene 164545 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

GP805

catalog :

164545

citations: 1

Reference
Desai P, Stuck M, Lv B, Pazour G. Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling. J Cell Biol. 2020;219: pubmed publisher

product information

Catalog Number :

164545

Product Name :

GP805

article :

doi	10.1083/jcb.201912104
id	28215746
pubmed_id	32435793

bacterial resistance :

Ampicillin

cloning :

backbone

Phage lentivirus

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Lentiviral

growth strain :

Expresses Smo-FLAG K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	GgCryD1
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	E130215L21Rik, Smoh, bnb, smoothened
gene	Smo
id	319757

genbank_ids

mutation

All 21 cytoplamic Lysines of Smoothened changed to Arginine. K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R

name

Smoothened K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R

shRNA_sequence

size

species

10090

Mus musculus