This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti sfGFP-LAMP1-mCherry (pHLARE)
catalog :
164478
citations: 1
Reference
Webb B, Aloisio F, Charafeddine R, Cook J, Wittmann T, Barber D. pHLARE: a new biosensor reveals decreased lysosome pH in cancer cells. Mol Biol Cell. 2021;32:131-142 pubmed publisher
product information
Catalog Number :
164478
Product Name :
pLenti sfGFP-LAMP1-mCherry (pHLARE)
article :
doi10.1091/mbc.E20-06-0383
id28215734
pubmed_id33237838
bacterial resistance :
Ampicillin
cloning :
backbonepLenti
backbone_mutation
backbone_origin
backbone_size6900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Please note there is a single mutation in sfGFP-D117Y. Depositor confirms this is not a concern for plasmid function.
growth strain :
Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. Plasmid for lentivirus production.
origin :
30
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV-F, GFP-F, mCherry-R
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesLGP120
geneLamp1
id25328
genbank_ids
mutation
nameLAMP1
shRNA_sequence
size2755
species
10116
Rattus norvegicus
tags
locationC terminal on insert
tagmCherry
locationN terminal on insert
tagsuperfolder GFP
resistance markers :
5293
tags :
Unknown
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA