This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA
catalog :
164471
citations: 1
Reference
Cheng L, Cring M, Wadkins D, Kuehn M. Absence of Connexin 43 Results in Smaller Retinas and Arrested, Depolarized Retinal Progenitor Cells in Human Retinal Organoids. Stem Cells. 2022;40:592-604 pubmed publisher
product information
Catalog Number :
164471
Product Name :
pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA
article :
doi10.1093/stmcls/sxac017
id28215728
pubmed_id35263762
bacterial resistance :
Ampicillin
cloning :
backbonepSpCas9(BB)-2A-Puro (PX459)
backbone_mutationInsertion of a sgRNA sequence targeting GJA1
backbone_originFeng Zhang
backbone_size9200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
A student in University of Iowa did this cloning.
growth strain :
Expresses sgRNA targeting human GJA1 locus
origin :
37
pi :
alt_names
CX43
cloning
clone_methodRestriction Enzyme
cloning_site_3BbsI
cloning_site_5BbsI
promoterU6
sequencing_primer_3
sequencing_primer_5GAGGGCCTATTTCCCATGATTCC
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesAVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA
geneGJA1
id2697
genbank_ids
NM_000165.5
mutation
nameGap junction alpha-1 (GJA1)
shRNA_sequenceAAGCCTACTCAACTGCTGGA
size
species
9606
Homo sapiens
tags
plasmid copy :
Matthew R. Cring
resistance markers :
5277
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA