MG15-gal (mut p53 binding sites) | Addgene 16447 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

MG15-gal (mut p53 binding sites)

catalog :

16447

citations: 1

Reference
El Deiry W, Tokino T, Velculescu V, Levy D, Parsons R, Trent J, et al. WAF1, a potential mediator of p53 tumor suppression. Cell. 1993;75:817-25 pubmed

product information

Catalog Number :

16447

Product Name :

MG15-gal (mut p53 binding sites)

article :

doi	10.1016/0092-8674(93)90500-P
id	1832
pubmed_id	8242752

bacterial resistance :

Ampicillin

cloning :

backbone

pBluescript II SK + bgal

backbone_mutation

backbone_origin

backbone_size

6200

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Other

beta-gal reporter

growth notes :

MG15-gal was cloned by inserting a HindIII-EcoRI fragment containing p53-binding elements (Kern et al Science 1992 May 8;256(5058):827-30) into the Hindlll-EcoRI sites of pBluescript II SK(+). A 200 bp EcoRI-BamHI fragment containing the polyoma promoter, and a 3kb beta-galactosidase cassette without promoter elements, were then cloned downstream. MG15 contains 15 copies of a mutated p53-binding sequence. Direct repeats of the oligonucleotide MG (5'-CCTTAATGGACTTTAATGG- 3') were used for the p53 nonbinding control sequences.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	HindIII
promoter
sequencing_primer_3
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BCC7, BMFS5, LFS1, P53, TRP53
gene	TP53
id	7157

genbank_ids

mutation

name

p53-binding site (mutant)

shRNA_sequence

size

species