MG15-luc (mut p53 binding sites) | Addgene 16443 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

MG15-luc (mut p53 binding sites)

catalog :

16443

citations: 7

Reference
Iwai M, Kajino T, Nakatochi M, Yanagisawa K, Hosono Y, Isomura H, et al. Long non-coding RNA TILR constitutively represses TP53 and apoptosis in lung cancer. Oncogene. 2022;: pubmed publisher
Lai S, Higuchi T, Tsuda M, Sugiyama Y, Morisawa K, Taniguchi T, et al. NF90-NF45 is essential for β cell compensation under obesity-inducing metabolic stress through suppression of p53 signaling pathway. Sci Rep. 2022;12:8837 pubmed publisher
Xing Y, Wei X, Liu Y, Wang M, Sui Z, Wang X, et al. Autophagy inhibition mediated by MCOLN1/TRPML1 suppresses cancer metastasis via regulating a ROS-driven TP53/p53 pathway. Autophagy. 2021;:1-23 pubmed publisher
Pelletier J, Riaño Canalias F, Almacellas E, Mauvezin C, Samino S, Feu S, et al. Nucleotide depletion reveals the impaired ribosome biogenesis checkpoint as a barrier against DNA damage. EMBO J. 2020;39:e103838 pubmed publisher
Yang L, Li Y, Bhattacharya A, Zhang Y. PEPD is a pivotal regulator of p53 tumor suppressor. Nat Commun. 2017;8:2052 pubmed publisher
O Driscoll C, Bressler J. Hairless expression attenuates apoptosis in a mouse model and the COS cell line; involvement of p53. PLoS ONE. 2010;5:e12911 pubmed publisher
El Deiry W, Tokino T, Velculescu V, Levy D, Parsons R, Trent J, et al. WAF1, a potential mediator of p53 tumor suppression. Cell. 1993;75:817-25 pubmed

product information

Catalog Number :

16443

Product Name :

MG15-luc (mut p53 binding sites)

article :

doi	10.1016/0092-8674(93)90500-P
id	1832
pubmed_id	8242752

bacterial resistance :

Ampicillin

cloning :

backbone

n/a

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

growth notes :

MG15-Luc was cloned by inserting a HindIII-EcoRI fragment containing p53-binding elements (Kern et al Science 1992 May 8;256(5058):827-30) into the Hindlll-EcoRI sites of pBluescript II SK(+). A 200 bp EcoRI-BamHI fragment containing the polyoma promoter, and a 2.6 kb SacI luciferase cassette without promoter elements, were then cloned downstream. MG15 contains 15 copies of a mutated p53-binding sequence. Addgene has confirmed the presence of 8 of these copies. Direct repeats of the oligonucleotide MG (5'-CCTTAATGGACTTTAATGG- 3') were used for the p53 nonbinding control sequences.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRV
cloning_site_5	EcoRV
promoter
sequencing_primer_3	LucNRev
sequencing_primer_5	M13pUC-Fwd
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	BCC7, BMFS5, LFS1, P53, TRP53
gene	TP53
id	7157

genbank_ids

mutation

name

p53-binding site (mutant)

shRNA_sequence

size

species