This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PG13-luc (wt p53 binding sites)
catalog :
16442
citations: 34
Reference
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Lai S, Higuchi T, Tsuda M, Sugiyama Y, Morisawa K, Taniguchi T, et al. NF90-NF45 is essential for β cell compensation under obesity-inducing metabolic stress through suppression of p53 signaling pathway. Sci Rep. 2022;12:8837 pubmed publisher
Haraoka Y, Akieda Y, Nagai Y, Mogi C, Ishitani T. Zebrafish imaging reveals TP53 mutation switching oncogene-induced senescence from suppressor to driver in primary tumorigenesis. Nat Commun. 2022;13:1417 pubmed publisher
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Islam M, Junod S, Zhang S, Buuh Z, Guan Y, Zhao M, et al. Unprotected peptide macrocyclization and stapling via a fluorine-thiol displacement reaction. Nat Commun. 2022;13:350 pubmed publisher
Wang B, Varela Eirin M, Brandenburg S, Hernandez Segura A, van Vliet T, Jongbloed E, et al. Pharmacological CDK4/6 inhibition reveals a p53-dependent senescent state with restricted toxicity. EMBO J. 2022;:e108946 pubmed publisher
Xing Y, Wei X, Liu Y, Wang M, Sui Z, Wang X, et al. Autophagy inhibition mediated by MCOLN1/TRPML1 suppresses cancer metastasis via regulating a ROS-driven TP53/p53 pathway. Autophagy. 2021;:1-23 pubmed publisher
Gudikote J, Cascone T, Poteete A, Sitthideatphaiboon P, Wu Q, Morikawa N, et al. Inhibition of nonsense-mediated decay rescues p53β/γ isoform expression and activates the p53 pathway in MDM2-overexpressing and select p53-mutant cancers. J Biol Chem. 2021;297:101163 pubmed publisher
Giebel N, de Jaime Soguero A, García Del Arco A, Landry J, Tietje M, Villacorta L, et al. USP42 protects ZNRF3/RNF43 from R-spondin-dependent clearance and inhibits Wnt signalling. EMBO Rep. 2021;22:e51415 pubmed publisher
Chi R, van der Watt P, Wei W, Birrer M, Leaner V. Inhibition of Kpnβ1 mediated nuclear import enhances cisplatin chemosensitivity in cervical cancer. BMC Cancer. 2021;21:106 pubmed publisher
Kang M, Tang B, Li J, Zhou Z, Liu K, Wang R, et al. Identification of miPEP133 as a novel tumor-suppressor microprotein encoded by miR-34a pri-miRNA. Mol Cancer. 2020;19:143 pubmed publisher
Kutle I, Szymańska de Wijs K, Bogdanow B, Cuvalo B, Steinbrück L, Jonjic S, et al. Murine Cytomegalovirus M25 Proteins Sequester the Tumor Suppressor Protein p53 in Nuclear Accumulations. J Virol. 2020;94: pubmed publisher
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Li Q, Hao Q, Cao W, Li J, Wu K, Elshimali Y, et al. PP2Cδ inhibits p300-mediated p53 acetylation via ATM/BRCA1 pathway to impede DNA damage response in breast cancer. Sci Adv. 2019;5:eaaw8417 pubmed publisher
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product information
Catalog Number :
16442
Product Name :
PG13-luc (wt p53 binding sites)
article :
doi10.1016/0092-8674(93)90500-P
id1832
pubmed_id8242752
bacterial resistance :
Ampicillin
cloning :
backbonepBluescript II SK + luc
backbone_mutation
backbone_origin
backbone_size5800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth notes :
PG13-Luc was cloned by inserting a HindIII-EcoRI fragment containing p53-binding elements (Kern et al Science 1992 May 8;256(5058):827-30) into the Hindlll-EcoRI sites of pBluescript II SK(+). A 200 bp EcoRI-BamHI fragment containing the polyoma promoter, and a 2.6 kb SacI luciferase cassette without promoter elements, were then cloned downstream. PG13 contains 13 copies of the p53-binding consensus sequence. Direct repeats of the oligonucleotide PG (5'-CCAGGCAAGTCCAGGCAGG-3') were used for the p53 binding sequences.
growth strain :
Luciferase reporter containing 13 copies of the p53-binding consensus sequence
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5HindIII
promoter
sequencing_primer_3LucNrev
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutation
namep53-binding site
shRNA_sequence
size
species
tags
locationC terminal on backbone
tagluciferase
resistance markers :
553
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA