This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV-Neo-Bam p53 R273H
catalog :
16439
citations: 20
Reference
Gao X, Zheng X, Zhang Y, Dong L, Sun L, Zhao N, et al. Deficient or R273H and R248W Mutations of p53 Promote Chemoresistance to 5-FU via TCF21/CD44 Axis-Mediated Enhanced Stemness in Colorectal Carcinoma. Front Cell Dev Biol. 2021;9:788331 pubmed publisher
Saini H, Sharma H, Mukherjee S, Chowdhury S, Chowdhury R. Verteporfin disrupts multiple steps of autophagy and regulates p53 to sensitize osteosarcoma cells. Cancer Cell Int. 2021;21:52 pubmed publisher
Lim J, Choi J, Park E, Choi Y. Interaction of promyelocytic leukemia/p53 affects signal transducer and activator of transcription-3 activity in response to oncostatin M. Korean J Physiol Pharmacol. 2020;24:203-212 pubmed publisher
Cai J, Xia J, Zou J, Wang Q, Ma Q, Sun R, et al. The PI3K/mTOR dual inhibitor NVP-BEZ235 stimulates mutant p53 degradation to exert anti-tumor effects on triple-negative breast cancer cells. FEBS Open Bio. 2020;10:535-545 pubmed publisher
Dabiri Y, Abu El Maaty M, Chan H, Wölker J, Ott I, Wolfl S, et al. p53-Dependent Anti-Proliferative and Pro-Apoptotic Effects of a Gold(I) N-Heterocyclic Carbene (NHC) Complex in Colorectal Cancer Cells. Front Oncol. 2019;9:438 pubmed publisher
Lakoduk A, Roudot P, Mettlen M, Grossman H, Schmid S, Chen P. Mutant p53 amplifies a dynamin-1/APPL1 endosome feedback loop that regulates recycling and migration. J Cell Biol. 2019;218:1928-1942 pubmed publisher
Brosnan Cashman J, Yuan M, Graham M, Rizzo A, Myers K, Davis C, et al. ATRX loss induces multiple hallmarks of the alternative lengthening of telomeres (ALT) phenotype in human glioma cell lines in a cell line-specific manner. PLoS ONE. 2018;13:e0204159 pubmed publisher
Yang Hartwich Y, Tedja R, Roberts C, Goodner Bingham J, Cardenas C, Gurea M, et al. p53-Pirh2 Complex Promotes Twist1 Degradation and Inhibits EMT. Mol Cancer Res. 2019;17:153-164 pubmed publisher
Schulz Heddergott R, Stark N, Edmunds S, Li J, Conradi L, Bohnenberger H, et al. Therapeutic Ablation of Gain-of-Function Mutant p53 in Colorectal Cancer Inhibits Stat3-Mediated Tumor Growth and Invasion. Cancer Cell. 2018;34:298-314.e7 pubmed publisher
Mircetic J, Dietrich A, Paszkowski Rogacz M, Krause M, Buchholz F. Development of a genetic sensor that eliminates p53 deficient cells. Nat Commun. 2017;8:1463 pubmed publisher
Ota A, Nakao H, Sawada Y, Karnan S, Wahiduzzaman M, Inoue T, et al. ?40p53? suppresses tumor cell proliferation and induces cellular senescence in hepatocellular carcinoma cells. J Cell Sci. 2017;130:614-625 pubmed publisher
Morton D, Patel D, Joshi J, Hunt A, Knowell A, Chaudhary J. ID4 regulates transcriptional activity of wild type and mutant p53 via K373 acetylation. Oncotarget. 2017;8:2536-2549 pubmed publisher
Karthikeyan S, Lantvit D, Chae D, Burdette J. Cadherin-6 type 2, K-cadherin (CDH6) is regulated by mutant p53 in the fallopian tube but is not expressed in the ovarian surface. Oncotarget. 2016;7:69871-69882 pubmed publisher
Laezza C, D Alessandro A, Di Croce L, Picardi P, Ciaglia E, Pisanti S, et al. p53 regulates the mevalonate pathway in human glioblastoma multiforme. Cell Death Dis. 2015;6:e1909 pubmed publisher
Labrie M, Vladoiu M, Grosset A, Gaboury L, St Pierre Y. Expression and functions of galectin-7 in ovarian cancer. Oncotarget. 2014;5:7705-21 pubmed
O Hainmhire E, Quartuccio S, Cheng W, Ahmed R, King S, Burdette J. Mutation or loss of p53 differentially modifies TGF? action in ovarian cancer. PLoS ONE. 2014;9:e89553 pubmed publisher
Campion C, Labrie M, Lavoie G, St Pierre Y. Expression of galectin-7 is induced in breast cancer cells by mutant p53. PLoS ONE. 2013;8:e72468 pubmed publisher
Saleh A, Savage J, Cao L, Soule B, Ly D, DeGraff W, et al. Cellular stress induced alterations in microRNA let-7a and let-7b expression are dependent on p53. PLoS ONE. 2011;6:e24429 pubmed publisher
Sauer L, Gitenay D, Vo C, Baron V. Mutant p53 initiates a feedback loop that involves Egr-1/EGF receptor/ERK in prostate cancer cells. Oncogene. 2010;29:2628-37 pubmed publisher
Baker S, Markowitz S, Fearon E, Willson J, Vogelstein B. Suppression of human colorectal carcinoma cell growth by wild-type p53. Science. 1990;249:912-5 pubmed
product information
Catalog Number :
16439
Product Name :
pCMV-Neo-Bam p53 R273H
article :
doi10.1126/science.2144057
id1828
pubmed_id2144057
bacterial resistance :
Ampicillin
cloning :
backbonepCMV-Neo-Bam
backbone_mutation
backbone_originVogelstein Lab
backbone_size6600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Note that there are multiple EcoRI sites in this plasmid besides those used for cloning.
origin :
37
pi :
alt_names
p53
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoter
sequencing_primer_3Bglob-pA-R
sequencing_primer_5Bglob-intron-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutationR273H
namep53 R273H
shRNA_sequence
size1300
species
9606
Homo sapiens
tags
resistance markers :
553
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA