This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV-Neo-Bam p53 R175H
catalog :
16436
citations: 21
Reference
Oak S, Karajgikar O, Teni T. Curcumin mediates selective aggregation of mutant p53 in cancer cells: A promising therapeutic strategy. Biochem Biophys Res Commun. 2023;677:141-148 pubmed publisher
Iwahashi N, Ikezaki M, Komohara Y, Fujiwara Y, Noguchi T, Nishioka K, et al. Cytoplasmic p53 aggregates accumulated in p53-mutated cancer correlate with poor prognosis. PNAS Nexus. 2022;1:pgac128 pubmed publisher
Palanikumar L, Karpauskaite L, Al Sayegh M, Chehade I, Alam M, Hassan S, et al. Protein mimetic amyloid inhibitor potently abrogates cancer-associated mutant p53 aggregation and restores tumor suppressor function. Nat Commun. 2021;12:3962 pubmed publisher
Tong X, Xu D, Mishra R, Jones R, Sun L, Schiltz G, et al. Identification of a druggable protein-protein interaction site between mutant p53 and its stabilizing chaperone DNAJA1. J Biol Chem. 2021;296:100098 pubmed publisher
Cai J, Xia J, Zou J, Wang Q, Ma Q, Sun R, et al. The PI3K/mTOR dual inhibitor NVP-BEZ235 stimulates mutant p53 degradation to exert anti-tumor effects on triple-negative breast cancer cells. FEBS Open Bio. 2020;10:535-545 pubmed publisher
Xu D, Tong X, Sun L, Li H, Jones R, Liao J, et al. Inhibition of mutant Kras and p53-driven pancreatic carcinogenesis by atorvastatin: Mainly via targeting of the farnesylated DNAJA1 in chaperoning mutant p53. Mol Carcinog. 2019;58:2052-2064 pubmed publisher
Aggarwal M, Saxena R, Asif N, Sinclair E, Tan J, Cruz I, et al. p53 mutant-type in human prostate cancer cells determines the sensitivity to phenethyl isothiocyanate induced growth inhibition. J Exp Clin Cancer Res. 2019;38:307 pubmed publisher
Dabiri Y, Abu El Maaty M, Chan H, Wölker J, Ott I, Wolfl S, et al. p53-Dependent Anti-Proliferative and Pro-Apoptotic Effects of a Gold(I) N-Heterocyclic Carbene (NHC) Complex in Colorectal Cancer Cells. Front Oncol. 2019;9:438 pubmed publisher
Yang Hartwich Y, Tedja R, Roberts C, Goodner Bingham J, Cardenas C, Gurea M, et al. p53-Pirh2 Complex Promotes Twist1 Degradation and Inhibits EMT. Mol Cancer Res. 2019;17:153-164 pubmed publisher
Mircetic J, Dietrich A, Paszkowski Rogacz M, Krause M, Buchholz F. Development of a genetic sensor that eliminates p53 deficient cells. Nat Commun. 2017;8:1463 pubmed publisher
Wang H, Wang Y, Wu D, Hung C, Chen C, Lee H. Targeting insulin-like growth factor-binding protein-3 by microRNA-125b promotes tumor invasion and poor outcomes in non-small-cell lung cancer. Tumour Biol. 2017;39:1010428317694316 pubmed publisher
Ota A, Nakao H, Sawada Y, Karnan S, Wahiduzzaman M, Inoue T, et al. ?40p53? suppresses tumor cell proliferation and induces cellular senescence in hepatocellular carcinoma cells. J Cell Sci. 2017;130:614-625 pubmed publisher
Morton D, Patel D, Joshi J, Hunt A, Knowell A, Chaudhary J. ID4 regulates transcriptional activity of wild type and mutant p53 via K373 acetylation. Oncotarget. 2017;8:2536-2549 pubmed publisher
Bado I, Nikolos F, Rajapaksa G, Gustafsson J, Thomas C. ERβ decreases the invasiveness of triple-negative breast cancer cells by regulating mutant p53 oncogenic function. Oncotarget. 2016;7:13599-611 pubmed publisher
Park E, Lee J, Park J, Bang S, Yi S, Kim B, et al. Transcriptional repression of cancer stem cell marker CD133 by tumor suppressor p53. Cell Death Dis. 2015;6:e1964 pubmed publisher
Chung J, Larsen A, Chen E, Bunz F. A PTCH1 homolog transcriptionally activated by p53 suppresses Hedgehog signaling. J Biol Chem. 2014;289:33020-31 pubmed publisher
Labrie M, Vladoiu M, Grosset A, Gaboury L, St Pierre Y. Expression and functions of galectin-7 in ovarian cancer. Oncotarget. 2014;5:7705-21 pubmed
Campion C, Labrie M, Lavoie G, St Pierre Y. Expression of galectin-7 is induced in breast cancer cells by mutant p53. PLoS ONE. 2013;8:e72468 pubmed publisher
Van Meter M, Mao Z, Gorbunova V, Seluanov A. SIRT6 overexpression induces massive apoptosis in cancer cells but not in normal cells. Cell Cycle. 2011;10:3153-8 pubmed
Sauer L, Gitenay D, Vo C, Baron V. Mutant p53 initiates a feedback loop that involves Egr-1/EGF receptor/ERK in prostate cancer cells. Oncogene. 2010;29:2628-37 pubmed publisher
Baker S, Markowitz S, Fearon E, Willson J, Vogelstein B. Suppression of human colorectal carcinoma cell growth by wild-type p53. Science. 1990;249:912-5 pubmed
product information
Catalog Number :
16436
Product Name :
pCMV-Neo-Bam p53 R175H
article :
doi10.1126/science.2144057
id1828
pubmed_id2144057
bacterial resistance :
Ampicillin
cloning :
backbonepCMV-Neo-Bam
backbone_mutation
backbone_originVogelstein Lab
backbone_size6600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Note that there are multiple EcoRI sites in this plasmid besides those used for cloning.
origin :
37
pi :
alt_names
p53
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3Bglob-pA-R
sequencing_primer_5Bglob-intron-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutationR175H
namep53 R175H
shRNA_sequence
size1300
species
9606
Homo sapiens
tags
resistance markers :
553
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA