pCMV-Neo-Bam p53 V143A | Addgene 16435 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCMV-Neo-Bam p53 V143A

catalog :

16435

citations: 5

Reference
Mircetic J, Dietrich A, Paszkowski Rogacz M, Krause M, Buchholz F. Development of a genetic sensor that eliminates p53 deficient cells. Nat Commun. 2017;8:1463 pubmed publisher
Bado I, Nikolos F, Rajapaksa G, Gustafsson J, Thomas C. ERÎ² decreases the invasiveness of triple-negative breast cancer cells by regulating mutant p53 oncogenic function. Oncotarget. 2016;7:13599-611 pubmed publisher
Campion C, Labrie M, Lavoie G, St Pierre Y. Expression of galectin-7 is induced in breast cancer cells by mutant p53. PLoS ONE. 2013;8:e72468 pubmed publisher
Sauer L, Gitenay D, Vo C, Baron V. Mutant p53 initiates a feedback loop that involves Egr-1/EGF receptor/ERK in prostate cancer cells. Oncogene. 2010;29:2628-37 pubmed publisher
Baker S, Markowitz S, Fearon E, Willson J, Vogelstein B. Suppression of human colorectal carcinoma cell growth by wild-type p53. Science. 1990;249:912-5 pubmed

product information

Catalog Number :

16435

Product Name :

pCMV-Neo-Bam p53 V143A

article :

doi	10.1126/science.2144057
id	1828
pubmed_id	2144057

bacterial resistance :

Ampicillin

cloning :

backbone

pCMV-Neo-Bam

backbone_mutation

backbone_origin

Vogelstein Lab

backbone_size

6600

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

A 1.8kb XbaI fragment encoding mutant p53 (from nucleotide -130 to 1671 relative to the ATG) was blunt-ended and ligated to BamHI linkers. This was then cloned into the unique BamHI site in the pCMV-Neo-Bam vector. This plasmid is also known as pC53-SCX3.

origin :

pi :

alt_names

p53

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	BamHI
promoter
sequencing_primer_3
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BCC7, BMFS5, LFS1, P53, TRP53
gene	TP53
id	7157

genbank_ids

mutation

V143A. Mutant cDNA from human colorectal tumor CX3.

name

p53 V143A

shRNA_sequence

size

1800

species

9606

Homo sapiens