This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV-Neo-Bam p53 wt
catalog :
16434
citations: 28
Reference
Meneghetti B, Wilson R, Dias C, Cadore N, Klamt F, Zaha A, et al. p53 mutants G245S and R337H associated with the Li-Fraumeni syndrome regulate distinct metabolic pathways. Biochimie. 2022;198:141-154 pubmed publisher
Gonzalez Rellan M, Fondevila M, Fernandez U, Rodríguez A, Varela Rey M, Veyrat Durebex C, et al. O-GlcNAcylated p53 in the liver modulates hepatic glucose production. Nat Commun. 2021;12:5068 pubmed publisher
Palanikumar L, Karpauskaite L, Al Sayegh M, Chehade I, Alam M, Hassan S, et al. Protein mimetic amyloid inhibitor potently abrogates cancer-associated mutant p53 aggregation and restores tumor suppressor function. Nat Commun. 2021;12:3962 pubmed publisher
Saini H, Sharma H, Mukherjee S, Chowdhury S, Chowdhury R. Verteporfin disrupts multiple steps of autophagy and regulates p53 to sensitize osteosarcoma cells. Cancer Cell Int. 2021;21:52 pubmed publisher
Kutle I, Szymańska de Wijs K, Bogdanow B, Cuvalo B, Steinbrück L, Jonjic S, et al. Murine Cytomegalovirus M25 Proteins Sequester the Tumor Suppressor Protein p53 in Nuclear Accumulations. J Virol. 2020;94: pubmed publisher
Long J, Galvan D, Mise K, Kanwar Y, Li L, Poungvarin N, et al. Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1. J Biol Chem. 2020;: pubmed publisher
Lim J, Choi J, Park E, Choi Y. Interaction of promyelocytic leukemia/p53 affects signal transducer and activator of transcription-3 activity in response to oncostatin M. Korean J Physiol Pharmacol. 2020;24:203-212 pubmed publisher
Mancikova V, Peschelova H, Kozlova V, Ledererova A, Ladungova A, Verner J, et al. Performance of anti-CD19 chimeric antigen receptor T cells in genetically defined classes of chronic lymphocytic leukemia. J Immunother Cancer. 2020;8: pubmed publisher
Xu Y, Xu Z, Li Q, Guo L, Wang Y, Zhou J, et al. Mutated p53 Promotes the Symmetric Self-Renewal of Cisplatin-Resistant Lung Cancer Stem-Like Cells and Inhibits the Recruitment of Macrophages. J Immunol Res. 2019;2019:7478538 pubmed publisher
Lin S, Yu L, Song X, Bi J, Jiang L, Wang Y, et al. Intrinsic adriamycin resistance in p53-mutated breast cancer is related to the miR-30c/FANCF/REV1-mediated DNA damage response. Cell Death Dis. 2019;10:666 pubmed publisher
Aggarwal M, Saxena R, Asif N, Sinclair E, Tan J, Cruz I, et al. p53 mutant-type in human prostate cancer cells determines the sensitivity to phenethyl isothiocyanate induced growth inhibition. J Exp Clin Cancer Res. 2019;38:307 pubmed publisher
Liu M, Wang Z, Liu Q, Zhu H, Xu N. Expression of Micro-RNA-492 (MiR-492) in Human Cervical Cancer Cell Lines is Upregulated by Transfection with Wild-Type P53, Irradiation, and 5-Fluorouracil Treatment In Vitro. Med Sci Monit. 2018;24:7750-7758 pubmed publisher
Luff S, Kao C, Papoutsakis E. Role of p53 and transcription-independent p53-induced apoptosis in shear-stimulated megakaryocytic maturation, particle generation, and platelet biogenesis. PLoS ONE. 2018;13:e0203991 pubmed publisher
Yang Hartwich Y, Tedja R, Roberts C, Goodner Bingham J, Cardenas C, Gurea M, et al. p53-Pirh2 Complex Promotes Twist1 Degradation and Inhibits EMT. Mol Cancer Res. 2019;17:153-164 pubmed publisher
Salari A, Thomay K, Lentes J, Ebersold J, Hagedorn M, Skawran B, et al. Effect of TP53 contact and conformational mutations on cell survival and erythropoiesis of human hematopoietic stem cells in a long term culture model. Oncotarget. 2018;9:29869-29876 pubmed publisher
Mircetic J, Dietrich A, Paszkowski Rogacz M, Krause M, Buchholz F. Development of a genetic sensor that eliminates p53 deficient cells. Nat Commun. 2017;8:1463 pubmed publisher
Mukherjee S, Dash S, Lohitesh K, Chowdhury R. The dynamic role of autophagy and MAPK signaling in determining cell fate under cisplatin stress in osteosarcoma cells. PLoS ONE. 2017;12:e0179203 pubmed publisher
Ros S, Flöter J, Kaymak I, da Costa C, Houddane A, Dubuis S, et al. 6-Phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 is essential for p53-null cancer cells. Oncogene. 2017;36:3287-3299 pubmed publisher
Karthikeyan S, Lantvit D, Chae D, Burdette J. Cadherin-6 type 2, K-cadherin (CDH6) is regulated by mutant p53 in the fallopian tube but is not expressed in the ovarian surface. Oncotarget. 2016;7:69871-69882 pubmed publisher
Kitade S, Onoyama I, Kobayashi H, Yagi H, Yoshida S, Kato M, et al. FBXW7 is involved in the acquisition of the malignant phenotype in epithelial ovarian tumors. Cancer Sci. 2016;107:1399-1405 pubmed publisher
Salari A, Thomay K, Himmler K, Vajen B, Schienke A, Hagedorn M, et al. Establishing a murine xenograft-model for long-term analysis of factors inducing chromosomal instability in myelodysplastic syndrome: Pitfalls and successes. Cancer Genet. 2016;209:258-66 pubmed publisher
Ho D, Kim H, Kim J, Sim H, Ahn H, Kim J, et al. Leucine-Rich Repeat Kinase 2 (LRRK2) phosphorylates p53 and induces p21(WAF1/CIP1) expression. Mol Brain. 2015;8:54 pubmed publisher
Escobar D, Hepp M, Farkas C, Campos T, Sodir N, Morales M, et al. Sall2 is required for proapoptotic Noxa expression and genotoxic stress-induced apoptosis by doxorubicin. Cell Death Dis. 2015;6:e1816 pubmed publisher
Chung J, Larsen A, Chen E, Bunz F. A PTCH1 homolog transcriptionally activated by p53 suppresses Hedgehog signaling. J Biol Chem. 2014;289:33020-31 pubmed publisher
O Hainmhire E, Quartuccio S, Cheng W, Ahmed R, King S, Burdette J. Mutation or loss of p53 differentially modifies TGF? action in ovarian cancer. PLoS ONE. 2014;9:e89553 pubmed publisher
Ferreira D, Afonso M, Rodrigues P, Simão A, Pereira D, Borralho P, et al. c-Jun N-terminal kinase 1/c-Jun activation of the p53/microRNA 34a/sirtuin 1 pathway contributes to apoptosis induced by deoxycholic acid in rat liver. Mol Cell Biol. 2014;34:1100-20 pubmed publisher
Sauer L, Gitenay D, Vo C, Baron V. Mutant p53 initiates a feedback loop that involves Egr-1/EGF receptor/ERK in prostate cancer cells. Oncogene. 2010;29:2628-37 pubmed publisher
Baker S, Markowitz S, Fearon E, Willson J, Vogelstein B. Suppression of human colorectal carcinoma cell growth by wild-type p53. Science. 1990;249:912-5 pubmed
product information
Catalog Number :
16434
Product Name :
pCMV-Neo-Bam p53 wt
article :
doi10.1126/science.2144057
id1828
pubmed_id2144057
bacterial resistance :
Ampicillin
cloning :
backbonepCMV-Neo-Bam
backbone_mutation
backbone_originVogelstein Lab
backbone_size6600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
A 1.8kb XbaI fragment encoding wild-type p53 (from nucleotide -130 to 1671 relative to the ATG) was blunt-ended and ligated to BamHI linkers. This was then cloned into the unique BamHI site in the pCMV-Neo-Bam vector. This plasmid is also known as pC53-SN3.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutation
namep53
shRNA_sequence
size1800
species
9606
Homo sapiens
tags
resistance markers :
553
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA