This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBSK-SN3 (wt p53)
catalog :
16421
citations: 1
Reference
Baker S, Fearon E, Nigro J, Hamilton S, Preisinger A, Jessup J, et al. Chromosome 17 deletions and p53 gene mutations in colorectal carcinomas. Science. 1989;244:217-21 pubmed
product information
Catalog Number :
16421
Product Name :
pBSK-SN3 (wt p53)
article :
doi10.1126/science.2649981
id1819
pubmed_id2649981
bacterial resistance :
Ampicillin
cloning :
backbonepBluescript SK
backbone_mutation
backbone_originStratagene
backbone_size3000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
A 1.8kb XbaI fragment of a cDNA clone containing wt p53 was cloned into the XbaI site of pBluescript SK. T7 polymerase should be used for in vitro transcription.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5XbaI
promoter
sequencing_primer_3T3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutation
namep53
shRNA_sequence
size1800
species
9606
Homo sapiens
tags
resistance markers :
553
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA