This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PKC beta I dNPS
catalog :
16379
citations: 2
Reference
Edens L, Levy D. cPKC regulates interphase nuclear size during Xenopus development. J Cell Biol. 2014;206:473-83 pubmed publisher
Soh J, Weinstein I. Roles of specific isoforms of protein kinase C in the transcriptional control of cyclin D1 and related genes. J Biol Chem. 2003;278:34709-16 pubmed
product information
Catalog Number :
16379
Product Name :
PKC beta I dNPS
article :
doi10.1074/jbc.M302016200
id1812
pubmed_id12794082
bacterial resistance :
Ampicillin
cloning :
backbonepHACE
backbone_mutation
backbone_origin
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
From user-provided sequence (see 'Reviews'), there are five amino acid differences from one of two NCBI reference sequences (AAA41868.1 and NP_001165776.1): 25A, 62F, 64K, 69C, and 294G. These mutations are also present in human, bovine, and xenopus sequences. An additional C-terminal mutation, H579Y, is present. These mutations are not believed to affect function.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPkcb, Prkcb1
genePrkcb
id25023
genbank_ids
mutationN-terminal pseudosubstrate domain deletion: aa 30-671
namePKC beta 1
shRNA_sequence
size1923
species
10116
Rattus norvegicus
tags
locationC terminal on backbone
tagHA
resistance markers :
569
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA