This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
TMD20
catalog :
163675
citations: 1
Reference
Sun X, Mahajan D, Chen B, Song Z, Lu L. A quantitative study of the Golgi retention of glycosyltransferases. J Cell Sci. 2021;134: pubmed publisher
product information
Catalog Number :
163675
Product Name :
TMD20
article :
doi10.1242/jcs.258564
id28215527
pubmed_id34533190
bacterial resistance :
Kanamycin
cloning :
backbonepmoxGFP-N1
backbone_mutationThe original NdeI in pmoxGFP-N1 is abolished.
backbone_originE. Snapp (Addgene plasmid # 68070)
backbone_size4695
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses a ST6GAL1 mutant with 20 amino acids in its TMD in mammalian cells
origin :
37
pi :
alt_names
ST6GAL1
ST
cloning
clone_methodRestriction Enzyme
cloning_site_3AgeI
cloning_site_5XhoI
promoter
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCDw75, SIAT1, ST6GalI, ST6N
geneST6GAL1
id6480
genbank_ids
BC040009
mutationFor this partial length ST chimera, two restriction sites, SpeI and NdeI, were introduced into the TMD by silent muation and its TMD length is elongated to 20 amino acids by inserting Ala, Val and Leu residues.
namepartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant
shRNA_sequence
size348
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagGFP
resistance markers :
3183
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA