SBP-ST*-GFP | Addgene 163668 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

SBP-ST*-GFP

catalog :

163668

citations: 1

Reference
Sun X, Mahajan D, Chen B, Song Z, Lu L. A quantitative study of the Golgi retention of glycosyltransferases. J Cell Sci. 2021;134: pubmed publisher

product information

Catalog Number :

163668

Product Name :

SBP-ST*-GFP

article :

doi	10.1242/jcs.258564
id	28215527
pubmed_id	34533190

bacterial resistance :

Kanamycin

cloning :

backbone

pmoxGFP-N1

backbone_mutation

The original NdeI in pmoxGFP-N1 is abolished.

backbone_origin

E. Snapp (Addgene plasmid # 68070)

backbone_size

4799

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Expresses SBP tagged partial length ST in mammalian cells

origin :

pi :

alt_names

ST6GAL1

cloning

clone_method	Restriction Enzyme
cloning_site_3	AgeI
cloning_site_5	XhoI
promoter
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CDw75, SIAT1, ST6GalI, ST6N
gene	ST6GAL1
id	6480

genbank_ids

BC040009

mutation

This chimera contains the first 113 amino acids of ST. Two restriction sites, SpeI and NdeI, were introduced into the TMD by silent muation.

name

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids)

shRNA_sequence

size

339

species

9606

Homo sapiens