This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pWZ157
catalog :
163639
citations: 1
Reference
Adikes R, Kohrman A, Martinez M, Palmisano N, Smith J, Medwig Kinney T, et al. Visualizing the metazoan proliferation-quiescence decision in vivo. elife. 2020;9: pubmed publisher
product information
Catalog Number :
163639
Product Name :
pWZ157
article :
doi10.7554/eLife.63265
id28215599
pubmed_id33350383
bacterial resistance :
Ampicillin
cloning :
backbonepAP088
backbone_mutation
backbone_originBob Goldstein/Ari Pani
backbone_size11077
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Worm Expression
CRISPR
growth notes :
GFP fusion to C. elegans pcn-1 driven under its own endogenous promoter. Plasmid is for CRISPR/Cas9-mediated single copy insertion at the ttTi4348 site on Chromosome I and can be paired with sgRNA plasmid pAP082
growth strain :
pcn-1>PCN-1::GFP expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I
origin :
37
pi :
alt_names
PCNA
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterpcn-1
sequencing_primer_3CAGGAAACAGCTATGACCATG
sequencing_primer_5tgtaaaacgacggccagt
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_W03D2.4
genepcn-1
id177161
genbank_ids
mutation
namepcn-1
shRNA_sequence
size3403
species
6239
Caenorhabditis elegans
tags
locationC terminal on insert
tagGFP
resistance markers :
5275
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA