This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
MSCV-BCL2
catalog :
163611
citations: 1
Reference
Caeser R, Di Re M, Krupka J, Gao J, Lara Chica M, Dias J, et al. Genetic modification of primary human B cells to model high-grade lymphoma. Nat Commun. 2019;10:4543 pubmed publisher
product information
Catalog Number :
163611
Product Name :
MSCV-BCL2
article :
doi10.1038/s41467-019-12494-x
id28207174
pubmed_id31586074
bacterial resistance :
Ampicillin
cloning :
backboneMSCV_IRES_huDC2
backbone_mutationBCL2 was cloned into MSCV_IRES_huDC2 using Gibson Assembly
backbone_origin
backbone_size6574
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
The huCD2 portion of the MSCV_IRES_huDC2 backbone was added by Dr Martin Turner, Babraham Institute, Cambridge by modification of Addgene plasmid # 27490.
growth strain :
Overexpression of human BCL2
origin :
37
pi :
alt_names
B-Cell CLL/Lymphoma 2
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterLTR
sequencing_primer_3CCTCACATTGCCAAAAGACG
sequencing_primer_5CTTTATCCAGCCCTCAC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBcl-2, PPP1R50
geneBCL2
id596
genbank_ids
NM_000633
mutation
nameBCL2
shRNA_sequence
size722
species
9606
Homo sapiens
tags
resistance markers :
4733
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA