This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
N-GFP-Dcp2
catalog :
163585
citations: 1
Reference
Xing W, Muhlrad D, Parker R, Rosen M. A quantitative inventory of yeast P body proteins reveals principles of composition and specificity. elife. 2020;9: pubmed publisher
product information
Catalog Number :
163585
Product Name :
N-GFP-Dcp2
article :
doi10.7554/eLife.56525
id28215589
pubmed_id32553117
bacterial resistance :
Ampicillin
cloning :
backbonepRP1903
backbone_mutation
backbone_origin
backbone_size5827
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
5' sequencing primers: Dcp2-seq1b:CGAATTTTACGTCTGAGGAAAG. Dcp2-seq2b: gacatagattgttgcattag. Dcp2-seq3b: cttcagcatttgaaagagca Dcp2-seq4b:gcaaaacaataatgatgaaa Dcp2-seq5b: gagaacaatttccaagacgg. Dcp2-seq7:tgaaacgcaacgacgctaca Dcp2-seq8b: GATACCCAGATCATATGAAACG
growth strain :
express N-terminal GFP tagged Dcp2 FL at endogenous level in S.cerevisiae
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterDCP2 promoter
sequencing_primer_3Dcp2-seq3: CCACATTGATAAGGGTCTC
sequencing_primer_5See comments
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYNL118C, PSU1
geneDCP2
id855605
genbank_ids
mutation
nameDcp2
shRNA_sequence
size4174
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on insert
tagGFP
plasmid copy :
received from Roy Parker's lab at University of Colorado, Boulder
resistance markers :
4315
tags :
Low Copy
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA