This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Dcp2 H1 WD
catalog :
163580
citations: 1
Reference
Xing W, Muhlrad D, Parker R, Rosen M. A quantitative inventory of yeast P body proteins reveals principles of composition and specificity. elife. 2020;9: pubmed publisher
product information
Catalog Number :
163580
Product Name :
Dcp2 H1 WD
article :
doi10.7554/eLife.56525
id28215589
pubmed_id32553117
bacterial resistance :
Ampicillin
cloning :
backbonepRP1903
backbone_mutation
backbone_origin
backbone_size5827
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Impaired Edc3 binding site (first HLM); impaired RNA cap recognition site (W50, D54). 5' sequencing primers: Dcp2-seq1b:CGAATTTTACGTCTGAGGAAAG. Dcp2-seq2b: gacatagattgttgcattag. Dcp2-seq3b: cttcagcatttgaaagagca Dcp2-seq4b:gcaaaacaataatgatgaaa Dcp2-seq5b: gagaacaatttccaagacgg. Dcp2-seq7:tgaaacgcaacgacgctaca Dcp2-seq8b: GATACCCAGATCATATGAAACG
growth strain :
express Dcp2 H1 WD at endogenous level in S.cerevisiae
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5SacI
promoterDCP2 promoter
sequencing_primer_3Dcp2-seq3: CCACATTGATAAGGGTCTC
sequencing_primer_5See comments
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYNL118C, PSU1
geneDCP2
id855605
genbank_ids
mutationW50A, D54A, L255A
nameDcp2
shRNA_sequence
size3438
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on insert
tagGFP
plasmid copy :
Roy Parker's lab at University of Colorado, Boulder
resistance markers :
4315
tags :
Low Copy
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA