This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
xfz8N/pCS2+
catalog :
16322
citations: 1
Reference
Deardorff M, Tan C, Conrad L, Klein P. Frizzled-8 is expressed in the Spemann organizer and plays a role in early morphogenesis. Development. 1998;125:2687-700 pubmed
product information
Catalog Number :
16322
Product Name :
xfz8N/pCS2+
article :
doi
id1799
pubmed_id9636083
bacterial resistance :
Ampicillin
cloning :
backbonepCS2+
backbone_mutation
backbone_origin
backbone_size4095
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Vertebrate expression
growth notes :
This construct contains the amino terminus of xfz8, which includes the signal sequence, cysteine-rich domain (CRD) and the linker region. It terminates just before the first transmembrane region. This plasmid does not contain a myc tag and is potent at causing enlarged cement gland and shortened axes. This construct was generated by removing a ClaI-XhoI fragment from Nxfz8/CS2MT. For sense transcripts, cut with NotI.
origin :
37
pi :
alt_names
xfz8N
Nxfz8
N8
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5SP6
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesXELAEV_18032854mg, FZD8, Fz-8, frizzled8, fz8, fzd8-a, fzd8-b, xfz8
genefzd8.S
id399367
genbank_ids
mutationExtracellular ligand binding domain of frizzled-8. Secreted wnt inhibitor.
namefrizzled-8
shRNA_sequence
size730
species
8355
Xenopus laevis
tags
resistance markers :
260
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA