Axin DeltaGID/pCS2MT | Addgene 16303 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Axin DeltaGID/pCS2MT

catalog :

16303

citations: 1

Reference
Hedgepeth C, Deardorff M, Rankin K, Klein P. Regulation of glycogen synthase kinase 3beta and downstream Wnt signaling by axin. Mol Cell Biol. 1999;19:7147-57 pubmed

product information

Catalog Number :

16303

Product Name :

Axin DeltaGID/pCS2MT

article :

doi
id	1789
pubmed_id	10490650

bacterial resistance :

Ampicillin

cloning :

backbone

pCS2+MT

backbone_mutation

backbone_origin

backbone_size

4352

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Vertebrate expression

growth notes :

For sense transcripts, cut with NotI and transcribe with SP6. GID= GSK3 interaction domain. NcoI digest should cut out a 1.733kb insert. Please note that the GSK interaction domain deletion begins at aa 321 when the sequence is compared to GenBank entry NM_001088405.1. As a result of the cloning process, there are two amino acids 'HM' prior to the end of the deletion at aa 504 that are not part of the endogenous axin sequence. Addgene's sequencing results also found a D559E mutation in the axin sequence that reflects a single nucleotide polymorphism. The depositing laboratory states that these differences are not a concern for the function of the plasmid.

origin :

pi :

alt_names

Axin

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	NcoI
promoter
sequencing_primer_3	T3
sequencing_primer_5	SP6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	XELAEV_18045581mg, axin1, fused
gene	axin1.L
id	398097

genbank_ids

mutation

Delta GID: deletion of GSK interaction domain aa 324-504

name

Axin DeltaGID

shRNA_sequence

size

species

8355

Xenopus laevis