This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Axin GID 1-2 LP/pCS2MT
catalog :
16300
citations: 1
Reference
Hedgepeth C, Deardorff M, Rankin K, Klein P. Regulation of glycogen synthase kinase 3beta and downstream Wnt signaling by axin. Mol Cell Biol. 1999;19:7147-57 pubmed
product information
Catalog Number :
16300
Product Name :
Axin GID 1-2 LP/pCS2MT
article :
doi
id1789
pubmed_id10490650
bacterial resistance :
Ampicillin
cloning :
backbonepCS2+MT
backbone_mutation
backbone_origin
backbone_size4352
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Vertebrate expression
growth notes :
L->P version has a single base pair change converting leu to pro. This mutant does not bind or inhibit GSK-3beta, does not activate LEF/tcf reporters, and does not dorsalize embryos. Based on Smalley et al (EMBO J). For sense transcripts, cut with NotI and transcribe with SP6
origin :
37
pi :
alt_names
Axin
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NcoI
promoter
sequencing_primer_3T3
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesXELAEV_18045581mg, axin1, fused
geneaxin1.L
id398097
genbank_ids
mutationGSK-3 interaction domain aa 320-429 with L to P mutation
nameAxin GID 1-2 LP
shRNA_sequence
size329
species
8355
Xenopus laevis
tags
locationN terminal on backbone
tag5x myc
resistance markers :
260
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA