This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Axin/pCS2MT
catalog :
16298
citations: 2
Reference
Bozatzi P, Dingwell K, Wu K, Cooper F, Cummins T, Hutchinson L, et al. PAWS1 controls Wnt signalling through association with casein kinase 1α. EMBO Rep. 2018;19: pubmed publisher
Hedgepeth C, Deardorff M, Klein P. Xenopus axin interacts with glycogen synthase kinase-3 beta and is expressed in the anterior midbrain. Mech Dev. 1999;80:147-51 pubmed
product information
Catalog Number :
16298
Product Name :
Axin/pCS2MT
article :
doi10.1016/S0925-4773(98)00203-2
id1790
pubmed_id10072781
bacterial resistance :
Ampicillin
cloning :
backbonepCS2+MT
backbone_mutation
backbone_origin
backbone_size4352
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Vertebrate expression
growth notes :
For sense mRNA, linearize with NotI and transcribe with SP6. For antisense probe, cut with Pst1, remove 3' overhang, and transcribe with T7. NcoI digest cuts out a 2333 bp fragment.
origin :
37
pi :
alt_names
Xaxin
Flaxin
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NcoI
promoter
sequencing_primer_3T3
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesXELAEV_18045581mg, axin1, fused
geneaxin1.L
id398097
genbank_ids
AF097313
mutation
nameAxin
shRNA_sequence
size2529
species
8355
Xenopus laevis
tags
locationN terminal on backbone
tag5x myc
resistance markers :
260
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA